Your Outsourcing Partner for Protein Research and Production Services

To meet the needs of our clients, Exon provides protein characterization of our expressed protein to insure the quality of recombinant protein.

Recombinant Protein Expression

Exon BioSystems is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

Antibody Engineering

Additional areas of expertise include assay development of G-protein coupled receptor, recombinant vaccine development and recombinant antibody production.

Biologics

Exon BioSystems is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

Protein Expression Services

Baculovirus Expression System

Baculovirus Expression Vector System (BEVS) has become a core technology for the production of high quality proteins. Due to its versatility and speed, this system is capable of generating large quantities of biologically active recombinant protein.


Pilot Trial

  • Gene synthesis or amplification of the gene of interest from customer-supplied vector and subcloning into a transfer vector with His-tag.
  • Transformation of E. coli with baculoviral DNA and gene of interest.
  • Transfection of SF9 or SF21 insect cells with bacmid.
  • Plaque purification and viral titer assay.
  • Evaluation and optimization of the expression level.

Time estimation: 6-8 weeks


Large scale expression and purification

  • Amplification of recombinant Baculovirus stock preparation.
  • Large-scale culture and infection of insect cells for protein expression (1-20L).
  • Cell pellet from insect culture will be lysed with our optimized protocol.Purification of recombinant protein from cell pellet or conditional media using affinity column.

Time estimation: 4-6 weeks

For more information please call _858-457-1920 or e-mail us at: info @ exonbio.com, or use our contact page: Contact