Your Outsourcing Partner for Protein Research and Production Services

To meet the needs of our clients, Exon BioSystems provides protein characterization of our expressed protein to insure the quality of recombinant protein.

Recombinant Protein Expression

Exon BioSystems is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

Antibody Engineering

Additional areas of expertise include assay development of G-protein coupled receptor, recombinant vaccine development and recombinant antibody production.

Biologics

Exon BioSystems is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

Molecular Biology Services

Induction Conditions

Protein expression is induced by the addition of the proper inducer or by changing the growth conditions. From this point on the cells will use most of their resources for the production of the target protein and will not grow much further.

  • The most commonly used promoters induction conditions are listed below.
Promoter induction typical condition range
trc (hybrid) IPTG 0.2 mM 0.05 - 2.0 mM
araBAD l-arabinose 0.2% 0.002 - 0.4 %
T7-lac op IPTG 0.2 mM 0.05 -2.0 mM
  • After induction the cultures are incubated from 3 hours to overnight depending on the induction temperature.
Incubation temperature incubation time
18°C overnight
30°C 5-6 h
37°C 3-4 h
For more information please call _858-457-1920 or e-mail us at: info @ exonbio.com, or use our contact page: Contact