Recombinant Protein Expression

Whether you are focused on lead discovery, production of targets for high throughput screenings, or characterization of interesting gene products or antibodies, our proprietary One-stop transient expression system will help optimize the expression and purification of your products.

Pilot Trial:

1. sub-clone your gene-of-interest into our proprietary mammalian expression vector. Codon optimization if necessary.

2. Midi- or Maxi-prep of plasmid DNA with endotoxin-free kit

3. Transfect 50ml-1 liter of either HEK293 or CHO serum-free suspension cells. Scale is dependent on customer's choice

4. Analyze expression products by western blot, ELISA or purification and quantify protein production.

Production Scale:

1. Giga plasmid DNA Prep with endotoxin-free kit

2. Transfection of HEK293 or CHO at scales from 1 liter to 100 liter as customer needs.

3. Culture 7-14 days depending on the cell viability

4. Harvest cells, clarification of supernatant, ultrafiltration to concentrate supernatant and to replace with purification compatible buffer.

5. Load onto the affinity column. Further polish the eluted protein if necessary.

6. Quality control, SDS-PAGE with coomassie blue staining or more if requested.