| Tag |
Size |
Type |
Comments |
| His-tag |
6, 8, or 10 aa |
Purification |
Both native or denature purification. |
| GST |
26 kD |
Purification |
native purification. Increase solubility. |
| Maltose binding Protein |
40 kD |
Purification and secretion |
Amylose affinity purification |
| Strep II |
8 aa |
Purification |
|
| FLAG |
8 aa |
Purification and detection |
Antibody purification, expensive. |
| Protein A |
14 kD |
Purification and secretion |
IgG affinity purification |
| DHFR |
25 kD |
Purification |
Methotrexate-agrose purification |
| Cellulose binding Domain |
107 aa |
Purification and secretion |
Cellulose based resin for affinity purification |
| Calmodulin binding protein |
4 kD |
Purification and detection |
Calmodulin/Ca2+ affinity purification |
| HA |
8 aa |
Purification and detection |
Antibody purification, expensive. |
| c-myc |
22 aa |
purification |
antibody purification, expensive. |
| NusA |
55 kD |
Enhance solubility |
Potentially improve solubility |
| Ubiquitin |
76 kD |
Enhance solubility |
Potentially improve solubility |
| GFP |
25 kD |
Detection |
Used as a report gene. |
| HSV |
11 aa |
Purification |
monoclonal antibody-based purification, denaturing low pH elution needed. |
