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Baculovirus Expression Vector System (BEVS) has become a core technology for the production of high quality proteins. Due to its versatility and speed, this system is capable of generating large quantities of biologically active recombinant protein.
Pilot trial
* Gene synthesis or amplification of the gene of interest from customer-supplied vector and subcloning into a transfer vector with His-tag
* Transformation of E. coli with baculoviral DNA and gene of interest.
* Transfection of SF9 or SF21 insect cells with bacmid.
* Plaque purification and viral titer assay.
* Evaluation and optimization of the expression level.
Time estimation: 6-8 weeks
Large scale expression and purification
* Amplification of recombinant Baculovirus stock preparation
* Large-scale culture and infection of insect cells for protein expression (1-20L).
* Cell pellet from insect culture will be lysed with our optimized protocol.Purification of recombinant protein from cell pellet or conditional media using affinity column
Time estimation: 4-6 weeks |
