Induction Conditions

Protein expression is induced by the addition of the proper inducer or by changing the growth conditions. From this point on the cells will use most of their resources for the production of the target protein and will not grow much further.

• The most commonly used promoters induction conditions are listed below.

• Promoter	induction	typical condition	range
  trc (hybrid)	IPTG	0.2 mM	0.05 - 2.0 mM
  araBAD	l-arabinose	0.2%	0.002 - 0.4 %
  T7-lac op	IPTG	0.2 mM	0.05 -2.0 mM

  .

  • After induction the cultures are incubated from 3 hours to overnight depending on the induction temperature.

    Incubation temperature	incubation time
    18°C	overnight
    30°C	5-6 h
    37°C	3-4 h

    
    

  Back to Bacterial Expression System

  Cotact us for further information info@exonbio.com or Call (858)457-1920