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From our experiences, more than half of our customers synthesize the gene for protein expression; therefore, codon optimization is the single most important factor in prokaryotc protein translation. The frequencies with which different codons are used vary significantly among different organisms, between abundant and rare proteins within the same organism. The codon optimized sequence perfectly fits the abundances of cognate tRNAs within the cell. Since a synthetic gene is designed as you wish, the codons used to encode different amino acids can be chosen to suit the expression host. There are numerous codon optimization algorisms available online. We have our gene algorithm to integrated codon optimization with respect to GC ratio, secondary structure and mRNA stability to maximize the translation efficiency.
Enhance heterologous protein expression
Optimize codon usage with the cell organism to be used to express the protein
Remove secondary structure and RNA unstable sequences.
Adjust GC content.,
Modify your gene to suit your needs:
1. Freely add or remove of restriction sites to facilitate gene manipulations.
2. Add N- or C-terminal tags for affinity purification, conjugation or modification.
3. Add linkers. ribosome binding sites, optimal Kozak consensus sequence, leading sequence , and introns
4. Freely add or remove any site for protein modifications, such as: phosphorylation sites, glycosylation sites, PEGylation sites.
ExonBio provides gene optimization at no extra charge.
Cotact us for further information info@exonbio.com
or Call (858) 457-1920
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