Bacterial system is a most often used expression system, its simple procedure and low cost made it very popular. ExonBio provides bacterial expression with following advantage:

•   One-step shopping from gene synthesis or gene cloning, expression and purification

•   Optimized expression with inducer, temperature and time courses.

•   High-throughput compatible.

Although bacterial expression system is the most popular system for protein expression, this system has its intrinsic defect. Many of the expressed proteins are not properly folded, therefore, aggregated in inclusion body.  Protein folding and unfolding have been extensively studied but the efficiency of in vitro folding is still limited. If your expressed protein is aggregated in inclusion body, other expression systems, such as mammalian expression system should be considered.

More Informatoins:

Optimization: Codon optimization and Codon plus strain

Optimization: Induction condition and temperature

Optimization: Solubility and refolding

Common: Host strains

Common: Promoters

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