Bacterial host is the most often used expression system due to its simple procedure and low cost, making it very popular. Exon BioSystems provides bacterial expression with the following advantage:

•   One-step shopping from gene synthesis or gene cloning, expression and purification

•   Optimized expression with inducer, temperature and time course.

•   High-throughput compatibility

Although the bacterial expression system is most popular for expressing a protein, this system has some intrinsic defects. Many of the eukaryotic expressed proteins are not properly folded, therefore, tend to aggregate into inclusion bodies.  Protein folding and unfolding have been extensively studied but the efficiency of in vitro folding is rather limited. If your expressed protein is aggregated into inclusion bodies, laternative expression systems, such as insect expression system and mammalian expression system should be considered.

More Informatoins:

Optimization: Codon optimization and Codon plus strain

Optimization: Induction condition and temperature

Optimization: Solubility and refolding

Common: Host strains

Common: Promoters

Comments: Advantage vs. disadvantage of bacterial expression system